Extended Data Fig. 14: PARP1 as an endogenous nuclear import shuttle to relocalize endogenous FKBP12.
From: Targeted protein relocalization via protein transport coupling
a, Representative live-cell images of partial endogenous FKBP12 localization upon treatment with different concentrations of TRAM 13 for 24 h. b, Violin plots illustrating a homogenous population shift in FKBP12 localization upon TRAM 13 treatment at different concentrations for 24 h. c, Representative live-cell images of endogenous FKBP12 localization after treatment with unlinked warheads 15 and 9 for 24 h. d, Quantification of localization changes observed in c. e, Violin plots showcasing the lack of homogenous population shift in FKBP12 localization when treated with the unlinked warheads 15 and 9 versus TRAM 13 for 24 h. f, Quantification of the change in FKBP12 localization upon treatment with 13 in competition with either 1x or 10x of 15 or 9 for 24 h. g, Representative images of FKBP12 localization in the competition assay in f. Images in a, c, g are representative of three biological replicates. Data in d, f are mean ± s.d. of three independent experiments. Scale bars are 20 µm. P values in b, d, e, f were determined by one-way ANOVA with Dunnett’s post hoc test comparing each condition to the DMSO control. Hoechst stains the nucleus.
