Extended Data Fig. 6: Exploration of mechanisms involved in Klebsiella reduction.
From: Commensal consortia decolonize Enterobacteriaceae via ecological control
a, Kp-2H7 was incubated in vitro under aerobic or anaerobic conditions with caecal suspensions from uncolonized GF mice or GF mice colonized with F31-, F18-, or F13-mix with or without prior heat-inactivation or filtration (0.22 μm). Kp-2H7 CFUs were counted after a 48 hr incubation at 37 °C in n = 3 biological replicates. Data are expressed as median ± IQR. b, GF mice (n = 3-5 per group) were colonized with Kp-2H7 and then treated with the indicated bacterial mix. Strains designated as F18-mix excluding A, B, C, or D are detailed in Fig. 1c. Caecal contents were collected on day 28 and subjected to targeted and non-targeted LC-MS/MS, GC-MS, or LC-QTOF/MS analyses. Heatmap depicts the z-score of each metabolite that showed a correlation with faecal Kp-2H7 CFUs (Pearson’s coefficient −0.6 > r > 0.6). 4-HBA, 4-hydroxybenzoic acid. c, Kp-2H7 was incubated with various chemical compounds at different concentrations in M9 medium under both aerobic and anaerobic conditions at 37 °C. Media supplemented with acetate or butyrate were adjusted to a final pH of 5.0 or 7.0 using NaOH. Bacterial growth was monitored by measuring absorbance at 600 nm every 0.5 hr using a microplate reader. Data are mean ± SEM from n = 3 biological replicates per condition. d, GF B6 mice were colonized with Kp-2H7 and subsequently treated with either F18-mix or F13-mix on day 0. From day 14, tributyrin (5 g/kg body weight) or a vehicle control was administered orally once daily for two weeks. Faecal CFUs of Kp-2H7 were counted through day 28 and are presented as median ± IQR. The day 28 data were compared using the Mann-Whitney U test (two-sided).
