Extended Data Fig. 8: The role of gluconate metabolism genes in Klebsiella growth.
From: Commensal consortia decolonize Enterobacteriaceae via ecological control
a, Wild-type (WT), ΔgntK, or ΔgntR Kp-2H7 strains were cultured at 37 °C for 48 hr in M9 minimal medium supplemented with the indicated carbohydrate (final concentration: 2mM). Bacterial growth was assessed by measuring absorbance at 600 nm. b, GF mice were inoculated with a 1:1 mixture of WT and ΔgntR Kp-2H7 and then treated with F18-mix or F13-mix. Faecal CFUs of Kp-2H7 were counted over time. Data are presented as median ± IQR, representative of two independent experiments, and were compared using the two-sided Mann-Whitney U test. c, WT, ΔgntK, or ΔgntR Kp-2H7 were cultured at 37 °C in M9 minimal medium supplemented with either glucose or gluconate (n = 3 biological replicates). Bacteria were collected during the early log phase (absorbance at 600 nm = 0.35) and subjected to RNA extraction and sequencing. The expression levels of genes involved in carbohydrate metabolism, expressed in transcripts per million (TPM), are presented as median ± IQR.
