Extended Data Fig. 4: Effects of F18-mix in an IBD model.
From: Commensal consortia decolonize Enterobacteriaceae via ecological control
a, b, GF B6 mice were colonized with faecal microbiota from either a patient with Crohn’s disease (CD#15) containing a high level of K. pneumoniae (a) or from a patient with ulcerative colitis (UC#5) containing ESBL+ E .coli (b). All mice were subsequently treated with vancomycin, and half received oral F18-mix administration four times over two days. CFUs of K. pneumoniae and E. coli (upper panels) and Shannon index (lower panels) of the faecal microbiota were examined longitudinally and compared by Mann-Whitney U test at day 28 (two-sided). c-g, GF Il10−/− mice were monocolonized with Kp-2H7, then orally administered the indicated bacterial mix seven days later. Representative haematoxylin and eosin staining of the colon (scale bar = 100 μm) (d), histological colitis scores (e), faecal lipocalin-2 and calprotectin levels (f), and frequency of IFNγ+ cells among colonic lamina propria CD4+TCRβ+ T cells (g) are shown. In panels a-c and e-g, median ± IQR are shown, representative of two independent experiments. Statistical analysis was performed using the Kruskal-Wallis test with the Benjamini-Hochberg correction for multiple comparisons.
