Extended Data Fig. 6: Structure: Tko FttA(H255A/H591A)–Spt4–Spt5–TEC44 and Tko FttA(H255A/H591A)–Spt4–Spt5–TEC52.
From: Structural basis of archaeal FttA-dependent transcription termination
a, Comparison of structures Tko FttA(H255A/H591A)–Spt4–Spt5–TEC44 (left) and Tko FttA(H255A/H591A)–Spt4–Spt5–TEC52 (right). Colours as in Fig. 2. b, Sequence alignment of regions of archaeal Spt5 that contact nontemplate-strand DNA in Tko FttA(H255A/H591A)–Spt4–Spt5–TEC44 and Tko FttA(H255A/H591A)–Spt4–Spt5–TEC52 to corresponding regions of yeast and human Spt5, Bacillus subtilis and Mycobacterium tuberculosis NusG (which have “pro-pausing” activity and which make corresponding protein-DNA interactions22,23), and E. coli NusG (which has “anti-pausing” activity and which does not make corresponding protein-DNA interactions21). Arrows, β-strands; helices, α-helices; red dots, residues that contact nontemplate-strand DNA in Tko FttA(H255A/H591A)–Spt4–Spt5–TEC44 and Tko FttA(H255A/H591A)–Spt4–Spt5–TEC52. Boxes denote conserved sequence positions. Colours denote levels of sequence conservation (red fill with white letters, high conservation; red letters, moderate conservation).
