Extended Data Fig. 7: Control experiments show that delayed dendritic CaMKII is specific to BTSP induction.
From: Dendritic, delayed, stochastic CaMKII activation in behavioural time scale plasticity
a-b, Top: Representative traces of CaMKII dendritic activity (smoothened by moving average of 60 points, 7.8 frames per second) of uncaging only and depolarization only experiments. Bottom: Lifetime change of all dendritic CaMKII activity for uncaging only and depolarization only. (shaded region, uncaging or depolarization protocol). c, Representative traces of smoothened 2dv-Camui dendritic recording from the same cells before (black) and after (orange) the BTSP protocol. (shaded region, BTSP protocol). d, Fraction of dendrites showing CaMKII activity in pre (no stim, n = 20) versus after BTSP (n = 20) induction. e, Peak amplitude of CaMKII activation in responsive BTSP (0.048 ± 0.005 ns, n = 82) and converse BTSP (0.057 ± 0.007 ns, n = 37) dendrites as compared to control dendrites- no-stim (0.038 ± 0.002 ns, n = 35), uncaging only (0.045 ± 0.008 ns, n = 26), and depolarization only (0.034 ± 0.003 ns, n = 22)). The data are presented as mean ± s.e.m. One-way ANOVA (F(4,121) = 1.223) followed by Dunnett’s post hoc test, BTSP vs cBTSP p = 0.73; BTSP vs uncage p = 0.99; BTSP vs depol p = 0.56; BTSP vs no stim p = 0.77. The number of dendrites is mentioned in the figure panels.
