Extended Data Fig. 8: Converse BTSP protocol induces delayed dendritic and stochastic CaMKII (DDSC).

a, Fluorescence lifetime images of 2dV-Camui expressing dendrite before, during and after the converse BTSP (cBTSP) protocol. “+” depicts the cBTSP stimulated spine. b, Representative EPSP traces from stimulated spines (10 recordings and average) before and after the cBTSP protocol. c, Time course of fluorescence lifetime changes in the stimulated spine and nearby dendrite from the dendrite in (b) (filtered) (shaded region, cBTSP protocol). d, Left: Representative dendritic 2dV-Camui recordings. Right: All 2dV-Camui recordings (filtered) during cBTSP induction. e, Relationship between the magnitude of potentiation and time of the first CaMKII peak after cBTSP protocol in a subset of neurons we could measure synaptic potentiation (Pearson R correlation, n = 14 pairs, r² = 0.02, p = 0.6). f, Simultaneous FLIM imaging of 2dV-Camui in soma and stimulated dendrite during cBTSP. g, Pie chart for 13 recordings, out of which 46% showed an increase in CaMKII activity in stimulated dendrites but not in the soma or primary dendrites. 31% of the recordings showed an increase in CaMKII activity in both the stimulated dendrite and the soma/primary dendrite, and 23% of the dendrites showed no CaMKII activity. h, Peak amplitude of CaMKII activation events in the stimulated dendrite compared with that in the soma or the primary dendrite. The data are presented as mean and individual values. Paired two-tailed t-test (t(9) = 2.35, p = 0.04). The number of dendrites are mentioned in the figure panels. *p < 0.05.