Extended Data Fig. 12: Characterization of delayed Ca²⁺ events in response to the BTSP protocol.

a, Representative dendritic shaft filled with Cal-590 dye showing a Ca²⁺ event. “+” depicts the BTSP stimulated spine. b, Representative dendritic Ca²⁺ traces after the BTSP protocol (pink shaded). The traces show multiple Ca²⁺ events after BTSP, in addition to smaller event during current injection of BTSP protocol. c, The frequency of Ca²⁺ events as a function of the time after BTSP protocol. The frequency peak appears around ~20-30 secs after the BTSP protocol. d, Ca²⁺ peak amplitude showed a smaller Ca²⁺ during depolarization and a significantly larger delayed Ca²⁺ peak amplitude. Data are presented as median and second quartile, along with individual values. Two-tailed unpaired t-test (t(92) = 3.2, p = 0.002). The number of dendrites is mentioned in the figure panels. ***p < 0.01.