Fig. 3: DDSC after BTSP induction.
From: Dendritic, delayed, stochastic CaMKII activation in behavioural time scale plasticity
a, Experimental set up (top), BTSP protocol (middle) and raw traces of EPSPs (ten recordings and average) before and after BTSP protocol (bottom). b, Fluorescence lifetime images (top) and time course (bottom) of a dendritic segment expressing 2dV-Camui during BTSP induction (shaded region, BTSP protocol). c, Representative traces (top) and all dendrites (bottom) for which fluorescence lifetime changes of 2dV-Camui were measured after BTSP (left) and no stimulation (no stim, right). Shaded region, BTSP protocol. d, Frequency of DDSC onsets after BTSP and cBTSP induction compared with uncaging only (uncage), current injection only (depol) and no stimulation controls. e, Cumulative frequency of d. Two-sided Kolmogorov–Smirnov test with Bonferroni’s correction, P = 0.00032 for BTSP versus uncage; P = 0.0000072 for BTSP versus depol; P = 0.00000026 for BTSP versus no stim; and P = 0.12 for BTSP versus cBTSP. f,g, Averaged time course (f) and area under the curve (30–40 s after BTSP) (g) of lifetime change of CaMKII activity under all five conditions. Data are presented as the mean ± s.e.m. in f and median and second quartile along with individual values in g. Two-way ANOVA (F4,100 = 5.434, P = 0.0005) with Dunnett’s multiple comparison test (all P values are shown in the source data file). NS, not significant. h, Fluorescence lifetime images of 2dV lifetime changes before (−2 s) and after (+50 s) BTSP protocol at secondary dendrites or soma. i, Representative 2dV-Camui traces of dendrite and of soma or primary dendrites from eight different cells. If the soma was not in the same z plane as the stimulated dendrite, the primary dendrite was used. j, Pie chart showing percentage of recordings that showed an increase in CaMKII activity either in both stimulated dendrite and soma (or primary dendrite) or specifically in the dendrites. k, Amplitude of DDSC in BTSP-induced dendrites and in soma or primary dendrites. The data are presented as the mean and individual values. Two-tailed paired t-test (t19 = 4.924, P = 0.000094). Plus symbol in images depicts the BTSP-stimulated spine. Number of dendrites are mentioned in the figure panels in parentheses wherever appropriate. *P < 0.05, ***P < 0.001. Scale bars, 2.5 μm (b) or 5 μm (h).
