Fig. 5: DDSC plays a crucial instructive role in BTSP.
From: Dendritic, delayed, stochastic CaMKII activation in behavioural time scale plasticity
a, Left, schematic of the photoactivatable CaMKII inhibitor paAIP2, which inhibits CaMKII after blue light (BL, 470 nm) exposure and becomes inactive within about 40 s when BL is stopped. Right, paAIP2-P2A-mEGFP-labelled CA1 neurons. Scale bar, 50 μm. b, Schematic of two separate CaMKII inhibition experiments shown in c, e and f. CaMKII was inhibited for 2 mins, 0 s (black) or 30 s (blue) after the BTSP protocol (orange). c, Representative EPSP traces of a stimulated spine (average of ten recordings) before and after BTSP induction in paAIP2-labelled neurons for which no BL stimulation was given (orange), or BL was given with 0 s delay (black) or 30 s delay (blue). d–g, Normalized time course (d–f) and summary of magnitude of EPSP potentiation (25–30 min) (g) in stimulated and adjacent spines for no BL (n = 17; d), BL with 0 s delay (n = 10; e), BL with 15 s delay (n = 10) or BL with 30 s delay (n = 11; f). Two way ANOVA (F7,68 = 4.94, P = 0.0001) with Tukey’s multiple comparison test (all P values are shown in the source data file). h, Top, BTSP protocol in voltage clamp (BTSPvc), for which 5 uncaging pulses (1 Hz) were paired with depolarization to 0 mV for 300 ms with 750 ms delay in the presence of TTX. Middle, protocol to artificially induce delayed CaMKII activity by applying additional long (20 s) depolarization 10–12 s after BTSPvc (BTSPvc+depol). Bottom, 5 uncaging pulses were paired with long depolarization 10–12 s after uncaging (uncage+depol). i, Representative EPSC traces of stimulated spine (average of 10 recordings) before and 20 min after BTSPvc, BTSPvc+depol and uncage+depol. j,k, Normalized EPSC amplitude time course (j) and summary of magnitude of potentiation (k) of stimulated and adjacent spines during BTSPvc (n = 10), BTSPvc+depol (stim, n = 19; adj, n = 11) and uncage+depol (stim, n = 14; adj, n = 13). Two-way ANOVA (F5,53 = 4.9, P = 0.0009) with Tukey’s multiple comparison test (all P values are shown in the source data file). The data in d–g, j and k are presented as the mean ± s.e.m. Number of cells are mentioned in the panels in parentheses wherever appropriate. Arrows depict BTSP, BTSPvc or uncaging induction. *P < 0.05, ***P < 0.001.
