Extended Data Fig. 1: BTSP protocol fails to induce plasticity in basal or distal synapses and is dependent on spiking, NMDAR and CaMKII.

a, Left: Representative basal dendritic image where BTSP was induced in one spine (+); Middle: Raw traces of EPSPs in a stimulated spine (10 recordings and average) before and after BTSP induction. Right: Averaged time course of normalized EPSP amplitude following BTSP induction in basal dendrites in stimulated and adjacent spines. Arrow depicts BTSP induction. b, Same as (a), but in distal dendrites. c, Left: Representative image of an apical dendritic shaft, where BTSP protocol was induced in one spine (+) in the presence of TTX (1 µM). All experiments were performed after incubating the slices for ~30 mins in TTX. Middle: Raw traces of EPSP in the stimulated spine (10 recordings and average) in TTX. Right: Averaged time course of normalized EPSP amplitude in TTX in stimulated and adjacent spines. d, Same as (c), but performed after incubating the slices for 30 mins in the presence of APV (25 µM). e, Same as (c), but the experiments were performed in the presence of QX314 (1-3 mM) in the patch pipette. f, Same as (c), but performed after incubating the slices for 30 mins in the presence of MK801 (10 µM). g, Same as (c), but in hippocampal slices made from Camk2a^T286A mice. The number of cells is mentioned in the figure panels in parentheses.