Extended Data Fig. 4: Gsdmd deficiency has little impact on the muscle microenvironment upon injury.
From: Gasdermin D-mediated metabolic crosstalk promotes tissue repair
a, Immunoblots of GSDMD and NINJ1 oligomerization (a) in injured TA muscle lysates (2dpi) after treatment with/without the membrane-impermeable BS3 crosslinker, which stabilizes protein-protein interactions for further analysis by immunoprecipitation. The intensity of Vinculin was used as control. b, Immunoblots of intracellular proteins using total muscle lysates and TIF to confirm the purity of the interstitial fluid. Ponceau S is used as loading control. c, OLINK analysis of muscle TIF. PCA plot of each sample during regeneration. d, Intramuscular levels of inflammatory and regenerating proteins determined by OLINK (n = 3 per group),and boxchart of intramuscular Il1β protein levels (n = 3). e, Immunoblots of HMGB1 secretion in muscle TIF at 2 dpi. f, Dotplot of pyroptosis index and indicated gene expression of three highly dynamic immune cell types. A representative (a,b,e) of at least two independent experiments is shown. Unpaired two-tailed t test applied for (d). Abbreviation: BS3, bis-(sulfosuccinimidyl)-suberate, used as a crosslinker.
