Extended Data Fig. 9: 11,12-EET has multi-organ pro-regenerating capacity.
From: Gasdermin D-mediated metabolic crosstalk promotes tissue repair
a, Mean CSA (n = 4), related to Fig. 6b. b, Representative H&E staining of TA muscle cross-sections with/without 11,12-EET supplement and the percentage of necrotic area at 14 dpi (n = 8). c, F4/80 immunofluorescence and statistics at 10 dpi (n = 7). d, Representative images of eMyHC+ myofibers and quantification (n = 4). e, The strength (n = 4) of TA muscles with indicated treatment post CTX-induced injury. f,g, Representative images (f left panel), the frequency distribution (f right panel) and mean of myofiber CSA (g) at 14 dpi with vehicle, vehicle+FGFR inhibitor, 11,12-EET or 11,12-EET + FGFR inhibitor treatment (n = 6). h-l, Representative H&E staining of corneal (h), statistics of epithelium and anterior stroma layer thickness (i) and inflammation infiltration (j) with 11,12-EET (50 ng 11,12-EET, twice daily) or vehicle treatment (n = 6). F4/80 staining (k) and quantification (n = 8) (l). m, Images of mice subjected to skin punch biopsy with 11,12-EET (300 ng daily) or vehicle treatment at 7 dpi. A representative of at least two independent experiments is shown. Unpaired two-tailed t test applied for (a-d,i,j,l); One-way ANOVA for (e,g) with tukey’s multiple comparisons test. Two-way ANOVA applied for (f) with Šidák correction. Mean ± SEM. Abbreviation: FGFRi, FGFR inhibitor; ROI, region of interest.
