Extended Data Fig. 10: 11,12-EET rejuvenates aged muscle.
From: Gasdermin D-mediated metabolic crosstalk promotes tissue repair
a-d, Schematic of UV-induced skin injury model (a). Statistics of wound closure over time (n = 10) (b), and images of mouse ear post UV irradiation with/without 11,12-EET (300 ng daily) treatment (n = 4) (c). Representative H&E staining of ears 14 days after UV irradiation (d). e, Quantification of EPHX2 expression in TA muscles from young and aged mice (n = 3)., related to Fig. 6l. f, Micrographs and quantification of collagen deposition in TA muscles of aged mice by Masson’s trichrome staining (n = 8). g, Quantification of muscle weight (n = 8) and strength (n = 4) in vehicle- and 11,12-EET-treated aged mice. h,i, Representative cross-sections of TA muscles stained with H&E (h) and myofiber CSA quantification (n = 8) i, Working model. A representative (c-i) or a pool (b) of at least two independent experiments is shown. Unpaired two-tailed t test applied for (e-h). Two-way ANOVA applied for (b) with Šidák correction; Mean ± SEM.
