Extended Data Fig. 7: Alternative confirmation of the TDP-43 glycine-rich region in heteromeric amyloid filaments from FTLD-TDP Type C.
From: Heteromeric amyloid filaments of ANXA11 and TDP-43 in FTLD-TDP type C
a, Cryo-EM reconstruction and atomic model of heteromeric amyloid filaments from FTLD-TDP Type C with an alternative conformation of the glycine-rich region, shown for single ANXA11 and TDP-43 molecules perpendicular to the helical axis. Cryo-EM density for TDP-43 is in grey and ANXA11 is in yellow. Buried ordered solvent is indicated with red dots. b, Overlay of the atomic models of filaments with the alternative conformation of the glycine-rich region with the main conformation (transparent). c, Amino acid sequence alignment of the secondary structure elements of TDP-43 in the filaments. Arrows indicate β-strands. C1, main conformation; C2, alternative conformation. d, Alignment of TDP-43 residues N295–R293 from the atomic models of FTLD-TDP Type C (pink) and Type A (cyan) filaments. a–c, The TDP-43 glycine-rich (G282–G310, magenta), hydrophobic (M311–S342, white) and Q/N-rich (Q343–Q345, green) regions are highlighted. ANXA11 is show in orange.
