Extended Data Fig. 8: Immunoblot and immunohistochemical analysis of ANXA11 and TDP-43 in the prefrontal cortex in FTLD-TDP.
From: Heteromeric amyloid filaments of ANXA11 and TDP-43 in FTLD-TDP type C
a, Immunoblot analysis of filament extracts from the prefrontal cortex of five individuals with FTLD-TDP Type C using antibodies against residues 3–15, 1–180 or 276–505 of ANXA11. A ~ 22 kDa ANXA11 NTF (white arrow) is observed for the antibodies against residues 3–15 and 1–180, but not 276–505. A minor population of full length ANXA11 (black arrow) is observed for all antibodies. b–d, Immunohistochemical analysis of prefrontal cortex sections from an additional five individuals with FTLD-TDP Type C (b), two with Type A (c) and two with Type B (d) using antibodies against pS409/410 TDP-43 and N-terminal ANXA11 (residues 1–180). Individual images for TDP-43 and ANXA11 are shown in greyscale to facilitate comparison, in addition to a merged image showing TDP-43 (green), ANXA11 (magenta) and DAPI (blue) staining. ANXA11 and TDP-43 colocalise with inclusions in the individuals with FTLD-TDP Type C, but only TDP-43 co-localises with the inclusions in the individuals with FTLD-TDP Types A and B. Scale bar, 40 µm.
