Fig. 4: Molecular pathology of ANXA11 in FTLD-TDP type C.
From: Heteromeric amyloid filaments of ANXA11 and TDP-43 in FTLD-TDP type C
a,b, Immunoblot analysis of filament extracts from the prefrontal cortex of individuals with FTLD-TDP types A (two individuals), B (two individuals) and C (six individuals) using antibodies to N-terminal ANXA11 (a; residues 1–180) and TDP-43-pS409/pS410 (b). An approximately 22-kDa ANXA11 NTF (white arrow in a) and a minor population of full-length ANXA11 (black arrow in a) are observed for all individuals with FTLD-TDP type C, but not for individuals with FTLD-TDP types A and B. Full-length TDP-43 (black arrow in b) and TDP-43 CTFs (black line in b) are observed for all individuals. c, Immunohistochemical analysis of prefrontal cortex sections from four individuals with FTLD-TDP type C using antibodies to TDP-43-pS409/pS410 and N-terminal ANXA11 (residues 1–180). Individual images for TDP-43 and ANXA11 are shown in greyscale to facilitate comparison, in addition to a merged image showing TDP-43 (green), ANXA11 (magenta) and DAPI (blue) staining. ANXA11 and TDP-43 colocalize with inclusions. Additional immunolabelling analyses are shown in Extended Data Figs. 8 and 9. Scale bar, 20 µm.
