Extended Data Fig. 7: Immunological and Haematological traits.
From: Dietary restriction impacts health and lifespan of genetically diverse mice
a, B cells (proportion of lymphocytes) by age (mean ± 2 s.e.; 5 months n = 936, 16 months n = 830, 28 months n = 485 mice) and by PLL as loess smooth with 95% confidence band (PLL:p = 9.85e-8, Diet:p = 2.42e-7, Diet x PLL:p = 0.751). b, Inflammatory monocytes (proportion of monocytes) by age (mean ± 2 s.e.; sample sizes as in panel a) and by PLL as loess smooth with 95% confidence band (PLL:p < 1.42e-7, Diet:p < 2.2e-16, Diet x PLL:p = 0.0148). c, Mature NK cells (proportion of NK cells) by age (mean ± 2 s.e.; sample sizes as in panel a) and by PLL as loess smooth with 95% confidence band (PLL:p < 2.2e-16, Diet:p < 4.08e-7, Diet x PLL:p = 0.0206). d, Eosinophils (proportion of myeloid cells) by age (mean ± 2 s.e.; sample sizes as in panel a) and by PLL as loess smooth with 95% confidence band (PLL:p < 2.2e-16, Diet:p < 6.93e-7, Diet x PLL:p = 0.210). e, Lifespan by Lymphocytes at 16 months (proportion of viable cells) with regression line, 95% confidence band, and diet-specific correlations (padj = 4.15e-15, Diet x Trait:p = 0.542, r = 0.234). f, Lifespan by effector CD4 T cells at 16 months (proportion of CD4 T cells) with regression line, 95% confidence band, and diet-specific correlations (padj = 1.70e-10, Diet x Trait:p = 0.481, r = −0.189). g, Haemoglobin (Hgb, g/dl) by age (mean ± 2 s.e.; 10 months n = 892, 22 months n = 665, 34 months n = 208 mice) and by PLL as loess smooth with 95% confidence band (PLL:p < 2.2e-16, Diet:p < 2.2e-16, Diet x PLL:p = 0.313). h, Red blood cell count (NumRBC, 106cells/ul) by age (mean ± 2 s.e.; sample sizes as in panel g) and by PLL as loess smooth with 95% confidence band (PLL:p < 2.2e-16, Diet:p < 2.2e-16, Diet x PLL:p = 0.999). i, Haematocrit (percent red cells) by age (mean ± 2 s.e.; sample sizes as in panel g) and by PLL as loess smooth with 95% confidence band (PLL:p < 2.2e-16, Diet:p < 2.2e-16, Diet x PLL:p = 0.508). j, Haemoglobin distribution width (HDW, cv) by age (mean ± 2 s.e.; sample sizes as in panel g) and by PLL as loess smooth with 95% confidence band (PLL:p < 2.2e-16, Diet:p = 3.83e-11, Diet x PLL:p = 1.57e-5). k, Lifespan by RDW at 22 months (cv) with regression line, 95% confidence band, and diet-specific correlations (padj = 1.65e-10, Diet x Trait:p = 0.745, r = −0.191). See Online Methods: Longitudinal Trait Analysis (panels a-d, g-j) and Trait Association with Lifespan (panels e, f, k) for statistical test details.
