Fig. 2: Body weight and composition effects on lifespan.
From: Dietary restriction impacts health and lifespan of genetically diverse mice
a, Lifetime trajectories of body weight (g) by diet groups. Data are monthly mean ± 1 s.e.m. n = 937. b, Body weight (g) trajectories as the running median (loess fit) by the PLL with 95% confidence bands. c, Diet-adjusted correlation with lifespan for body weight, change in body weight across three-month intervals (delta BW), LTM and FTM. The asterisks indicate multiple-testing-adjusted significance, determined by linear regression of lifespan on traits; *Padj < 0.01, **Padj < 0.001, ***Padj < 0.0001. d, The expected difference in lifespan per gram of body weight at ages 2 to 6 months and 18 months by diet. Data are mean ± 2 s.e.m. n = 937 (2 to 6 months) and n = 802 (18 months). e, Kaplan–Meier survival curves by diet group (colour) for mice below and above the median 6 month body weight. The dashed lines indicate the median survival times. Significance was determined using log-rank tests comparing diet within body weight strata. n = 469 (light) and n = 468 (heavy). f, Lifespan by change in body weight during the phenotyping period (10 to 11 months; PhenoDelta), showing the regression line, 95% confidence bands and diet-specific correlations (Padj < 2.2 × 10−16; diet × trait: P = 0.00172, r = 0.287). g, LTM (g) by age (mean ± 2 s.e.m.; n = 895 (10 months), n = 689 (22 months), n = 241 (34 months) mice) and by PLL as loess smoothing with the 95% confidence band (PLL: P < 2.2 × 10−16; diet: P < 2.2 × 10−16; diet × PLL: P = 1.07 × 10−7). h, FTM (g) by age (mean ± 2 s.e.m.; n values are as shown in g) and by PLL as loess smoothing with the 95% confidence bands (PLL: P < 2.2 × 10−16, diet: P < 2.2 × 10−16, diet × PLL: P = 0.168). For g and h, details of the statistical tests are provided in the ‘Longitudinal trait analysis’ section of the Methods.
