Fig. 3: Health and metabolic traits change with age and diet but are poor predictors of lifespan.
From: Dietary restriction impacts health and lifespan of genetically diverse mice
a,b, Heat maps of diet- and body-weight-adjusted correlation with lifespan for selected health (a) and metabolic (b) traits at annual testing intervals. Ages (1 year, 2 years and 3 years indicated as Y1, Y2 and Y3, respectively) vary depending on the assay (Supplementary Table 4). The asterisks indicate multiple-testing-adjusted significance, determined by linear regression of lifespan on traits; *Padj < 0.01. c, FI score (adjusted for technician and coat colour) by age (mean ± 2 s.e.m.; n = 770 (5 months), n = 909 (10 months), n = 834 (16 months), n = 704 (22 months), n = 489 (28 months), n = 260 (34 months) mice) and by PLL as loess smoothing with the 95% confidence bands (PLL: P < 2.2 × 10−16; diet: P = 7.37 × 10−5; diet × PLL: P = 0.633). d, Lifespan by frailty score (adjusted for technician and coat colour) at 28 months with regression line and 95% confidence band (Padj = 0.00238; diet × trait: P = 0.260, r = −0.90). e, Body temperature (°C; adjusted for coat colour) by age (mean ± 2 s.e.m.; sample sizes were as described in c) and by PLL as loess smoothing with the 95% confidence bands (PLL: P < 2.2 × 10−16; diet: P < 2.2 × 10−16; diet × PLL: P = 0.0242). f, Fasting glucose (mg dl−1, adjusted for body weight) by age (mean ± 2 s.e.m.; samples sizes were as described in c) and by PLL as loess smoothing with the 95% confidence bands (PLL: P < 2.80 × 10−4; diet: P < 2.2 × 10−16; diet × PLL: P = 0.0368). Statistical details are provided in the ‘Longitudinal trait analysis’ (c,e,f) and ‘Trait association with lifespan’ (d) sections of the Methods. BMD, bone mineral density; EE, energy expenditure; RQ, respiratory quotient.
