Fig. 4: Immune and haematologic traits change with age, respond to diet and predict lifespan.
From: Dietary restriction impacts health and lifespan of genetically diverse mice
a,b, Diet- and body-weight-adjusted correlation with lifespan for selected haematological traits from flow cytometry (a) and immune traits from complete blood counts (b) at the indicated ages. The asterisks indicate multiple-testing-adjusted significance, determined using linear regression of lifespan on traits; *Padj < 0.01, **Padj < 0.001, ***Padj < 0.0001. c, Lymphocytes (the proportion of viable cells) by age (mean ± 2 s.e.m.; n = 936 (5 months), n = 830 (16 months), n = 485 (28 months) mice) and by PLL as loess smoothing with the 95% confidence bands (PLL: P < 2.2 × 10−16; diet: P < 4.43 × 10−8; diet × PLL: P = 0.0670). d, Effector CD4 T cells (proportion of all CD4 T cells) by age (mean ± 2 s.e.m.; sample sizes were as described in c) and by PLL as loess smoothing with the 95% confidence bands (PLL: P < 2.2 × 10−16; diet: P = 0.268; diet × PLL: P = 0.300). e, RDW (coefficient of variation (cv)) by age (mean ± 2 s.e.m.; n = 892 (10 months), n = 665 (22 months), n = 208 (34 months) mice) and by PLL as loess smoothing with the 95% confidence bands (PLL: P < 2.2 × 10−16; diet: P < 2.2 × 10−16; diet × PLL: P = 4.77 × 10−5). f, Lifespan by RDW at 10 months, with the regression line, 95% confidence bands and diet-specific correlations (Padj = 5.71 × 10−11; diet × trait: P = 0.395, r = −0.239). Details of the statistical tests are provided in the ‘Longitudinal trait analysis’ (c–e) and ‘Trait association with lifespan’ (f) sections of the Methods. CLL, chronic lymphocytic leukaemia.
