Extended Data Fig. 3: Statistical analysis of physiological traits.
From: Dietary restriction impacts health and lifespan of genetically diverse mice
a, Overview of the phenotyping schedule: metabolic cages (MetCage), grip strength and frailty exams including body temperature, immune cell profiling by flow cytometry (FLOW), fasted blood glucose, home cage wheel running, voiding assay, rotarod, body composition by dual energy x-ray absorption (DEXA), echocardiogram (Echo), acoustic startle (AS), and complete blood count (CBC). Assays from Wheel to AS constitute the one-month-long intensive phenotyping period. b, Barplots showing the number of traits with significant (padj <0.01) associations with body weight (BW), diet, proportion of life lived (PLL), and diet x PLL interaction. Traits were categorized as health, metabolism, haematology, or immune (see Supplementary Table 6 and Online Methods: Longitudinal Trait Analysis for statistical test details). c, Barplots showing the number of traits that were significantly associated with lifespan after accounting for diet group and body weight. Ages (designated Y1, Y2, Y3) vary depending on the assay (see Supplementary Table 4). For traits with multiple measurements each year, we counted the most significant result (see Supplementary Table 7 and Online Methods: Trait Association with Lifespan for statistical test details). d, Volcano plot showing diet- and BW-adjusted correlations of physiological traits with lifespan vs. statistical significance (-log10p). e, Dendrogram shows hierarchical clustering (complete linkage, absolute correlation distance) of 50 traits with the most significant lifespan association. Trait names are shown as Year_AssayType_TraitName as defined in Supplementary Table 5.
