Extended Data Fig. 9: Visual representation changes induced by sparse viral SEP-GluA2 expression in PV interneurons.
From: Calcium-permeable AMPA receptors govern PV neuron feature selectivity
a, AAV-infected layer 2/3 PV interneurons within the monocular V1 area. Scale bar, 50 μm. b, The fraction of neurons with significant visual responses in each group (n = 325/267/161 total neurons from 7/7/5 mice, χ2 = 11.09, dF = 2, P = 0.0039, Chi-square test). c, The average response amplitude (∆F/F, mean ± SEM) of each group was not statistically different (n = 252/184/132 neurons, \({{\rm{\chi }}}_{(2)}^{2}\) = 0.5550, P = 0.7578, KW 1-way ANOVA). d-f, Waterfall plots displaying the overall visual response profile of the population of visually responsive neurons with positive preferred stimulus responses in the (d) PV-Cre;AAV-DIO-eGFP, (e) PV-Cre;AAV-DIO-SEP-GluA2, (f) PV-Cre;AAV-DIO-SEP-GluA2Q groups. g-j, Mouse-level statistics confirm that removal of CP-AMPARs in PV interneurons increases feature selectivity in layer 2/3 of mouse visual cortex. g, The SEP-GluA2 group displayed higher orientation selectivity compared to eGFP controls, whereas the SEP-GluA2Q group did not show increased OSI (n = 7/7/5 mice, P = 0.0401, one-way ANOVA; P = 0.0385 for eGFP vs. SEP-GluA2, P = 0.8307 for eGFP vs. SEP-GluA2Q, Tukey’s multiple comparison correction). h, Similarly, the SEP-GluA2 group displayed higher direction selectivity compared to the eGFP control group, whereas the SEP-GluA2Q group failed to show higher DSI (n = 7/7/5 mice, P = 0.0348, one-way ANOVA; P = 0.0318 for eGFP vs. SEP-GluA2, P = 0.7699 for eGFP vs. SEP-GluA2Q). i, Fraction of neurons with statistically significant visual response in each group were not different (0.86, 0.76, 0.89; n = 7/7/5 mice, P = 0.2247, one-way ANOVA). j, Average preferred response amplitudes (∆F/F) of each group were not statistically different (n = 7/7/5 mice, P = 0.7319, one-way ANOVA). k, The SEP-GluA1Q582R group showed a significantly higher OSI (mean ± SEM) compared to the SEP-GluA1 group (n = 42/49 neurons from 44/68 total neurons from 3/3 mice, P < 0.0001, unpaired t-test). l, The DSI (mean ± SEM) was not significantly different (P < 0.1648, Mann-Whitney U-test). m, The fraction of neurons with significant visual responses in each group was different (0.95/0.79; n = 44/68 total neurons from n = 3/3 mice, χ2 = 5.615, dF = 1, P = 0.0178, Chi-square test). n, The average response amplitude (∆F/F) of each group (mean ± SEM) was not significantly different (n = 42/49 neurons from 3/3 mice, P = 0.6076, Mann-Whitney U-test).
