Extended Data Fig. 12: FACS-assisted bulk RNA-seq of cortical PV interneurons: synaptic gene expression.
From: Calcium-permeable AMPA receptors govern PV neuron feature selectivity
a, Gene expression in PV interneurons of each genotype was statistically analyzed with DESeq2 and plotted using TPM (transcripts per million) to visualize expression levels. PV interneurons in PV-Cre;lsl-GFP-GluA2 mice display largely unchanged expression (mean values ± SEM) of major glutamate receptor genes compared to PV-Cre;lsl-eGFP control mice, with the exception of Gria2, which is overexpressed by roughly 2-fold in these mice (n = 9/7 mice; Wald test, Benjamini-Hochberg adjusted P < 0.0001). This is in line with the protein level increases (Fig. 1b) and matches the Gria2 expression level of typical excitatory neurons. b-c, AMPAR/GABAR interactor/postsynaptic regulator genes also display unaltered expression.
