Extended Data Fig. 14: Visual representation changes in excitatory neurons induced by global GluA2 homozygous knockout.

a, Two-photon micrograph of AAV-infected layer 2/3 excitatory neurons within monocular V1. Scale bar, 50 μm. b, The fraction of neurons with statistically significant visual responses in each group was not different (0.85/0.86; n = 739/500 neurons from n = 3/3 mice, χ² = 0.0292, dF = 1, P = 0.8644, Chi-square test). c, The average response amplitude (∆F/F) of each group was not significantly different (n = 504/340 neurons; P = 0.4060, Mann-Whitney U-test). Data are presented as mean values ± SEM. d, e, Waterfall plots displaying the overall visual response profile of the population of visually responsive neurons with positive preferred stimulus responses in the (d) GluA2-WT (+/+), (e) GluA2-KO (−/−) groups. f-i, Mouse-level statistics confirm that homozygous knockout of GluA2 lowers excitatory neuron orientation selectivity in layer 2/3 of mouse visual cortex. f, Quantification of orientation selectivity shows a significantly lower OSI in GluA2 knockouts compared to littermate wildtype (WT) controls (n = 3/3 mice, P = 0.0288, unpaired t-test). g, The GluA2-KO group displays a trend towards lower direction selectivity (n = 3/3 mice, P = 0.0540, unpaired t-test). h, The fraction of neurons with statistically significant visual responses in each group was not different. (n = 3/3 mice, P = 0.5605, unpaired t-test). i, The average response amplitude (∆F/F) of each group was not significantly different (n = 3/3 mice, P = 0.5144, unpaired t-test). Responses are plotted as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n.s., not significant, P ≥ 0.05.