Extended Data Fig. 3: Selective low expression of GluA2 and Gria2 in PV and SST interneurons in mice, marmosets, and humans.
From: Calcium-permeable AMPA receptors govern PV neuron feature selectivity
a, Immunohistochemical staining of PV and GluA2 in visual cortex layer 2/3. PV interneurons (asterisks) show markedly lower GluA2 expression compared to CaMKIIα excitatory counterparts. Layer 2/3 of visual cortex, scale bars, 10 μm. b, Quantification of relative GluA2 expression as a ratio of PV/CaMKIIα neurons (mean ± SEM) shows that PV interneurons express significantly less GluA2 (PV: 0.62 ± 0.03-fold vs CaMKIIα; n = 15 neuron pairs from 3 slices, 3 mice; P = 1.605x10−8, 1-sample t-test). c, GluA2 expression in PV interneurons and CaMKIIα excitatory neurons in the marmoset cortex. Scale bars, 10 μm. d, Marmoset PV interneurons express significantly less GluA2 compared to nearby CaMKIIα neurons (PV: 0.63 ± 0.03-fold vs CaMKIIα; n = 22 pairs from 7 slices, 3 marmosets, P = 1.062x10−10, one sample t-test). Bars and error bars denote mean ± SEM. e-g. High expression of GluA1 and low expression of GluA2 protein in PV interneurons. e, Immunohistochemical staining of PV, GluA1, and GluA2 in layer 2/3 of mouse visual cortex. PV interneurons (arrows) show markedly lower GluA2 expression, and higher GluA1 expression compared to all other neurons (all GluA1+ or GluA2+ and DAPI+ cells). Scale bar, 100 μm. f, Quantification of GluA1 expression (mean ± SEM) shows that PV interneurons express significantly more GluA1 (other neurons: 1.00 ± 0.02, n = 203 neurons/3 slices/3 mice; PV interneurons: 1.68 ± 0.10, n = 33 neurons; P = 2.319−25, unpaired t-test). g, Quantification of GluA2 expression (mean ± SEM) shows that PV interneurons express significantly less GluA2 (other neurons: 1.00 ± 0.02; PV interneurons: 0.66 ± 0.04; P = 5.861x10−9, unpaired t-test).
