Fig. 3: Remodelling of global chromatin conformation during human brain development.
From: Temporally distinct 3D multi-omic dynamics in the developing human brain
a, k-means clustering analysis groups single-cell 3C profiles by the distance distribution between interacting loci. b, Merged chromatin interaction profiles of the odd-numbered clusters identified in a. c, Cell-type-specific enrichments of clusters identified in a. EC, endothelial cell; PC, pericyte; VLMC, vascular leptomeningeal cell. d–f, Comparison of SE, LE and INT chromatin conformation found in single brain cells (d), bulk Hi-C profiles of diverse human tissues (e) and isolated neuronal and glial nuclei from primary adult human brain specimens (f). The vertical dashed lines indicate the threshold that separates short-range (coloured in orange) from long-range interactions (coloured in grey). a.u., arbitrary units. g,h, Remodelling of global chromatin conformation during the differentiation of upper-layer excitatory neurons (Exc-L1-3-CUX2) (g) and astrocytes (h) from the common RG-1 progenitor. i, Merged chromatin interaction profiles of developing cell populations across the differentiation of upper-layer excitatory neurons and astrocytes.
