Extended Data Fig. 3: In situ validation of cell-type marker genes predicted by CG methylation patterns.
From: Temporally distinct 3D multi-omic dynamics in the developing human brain
(a) UMAP of brain cells derived from late-gestational HPC samples. (b) UMAP showing TLL1 CG hypomethylation for more matured granule neurons. (c) UMAP showing TRPS1 CG hypomethylation in Mossy Cells and partially in CA3 neurons. (d) UMAP showing LRIG1 hypomethylation in astrocytes. (e) single molecular RNA in situ detection of TLL1, PROX1, and RBFOX3 transcripts in the hippocampus in the third trimester (GW 30 GW). (f) single molecular RNA in situ detection of TPRS1, GAD1, and RBFOX3 transcripts in the hippocampus in the third trimester. (g) single molecular RNA in situ detection of LRIG1 and ALDH1L1 transcripts in the hippocampus in the third trimester. (h) Quantification of LRIG1/ALDH1L1 co-expression (N = 4 imaging fields of view).
