Fig. 1: Gp54 in Bas11 is an activator of the CapRelSJ46 defence system.
From: A bacterial immunity protein directly senses two disparate phage proteins
a, Serial, tenfold dilutions of the indicated phages spotted on lawns of cells harbouring an empty vector (EV) or plasmid producing CapRelSJ46. Relative phage concentration is indicated by the height of the wedge. b, Serial dilutions of six escape clones of Bas11 and a control wild-type (WT) phage spotted on lawns of cells harbouring either an empty vector or a CapRelSJ46 expression vector, with the corresponding mutations in gene 54 labelled. c, Schematic of the gene 54 genomic region in Bas11, with mutations in the escape clones from b labelled. d, Cell viability assessed by serial dilutions of cells producing CapRelSJ46 from its native promoter, and the indicated variant of Gp54Bas11 from an arabinose-inducible promoter (Para) on medium containing glucose (Glu) or arabinose (Ara). e, In vitro transcription–translation assays using DHFR production from a DNA template as readout. Purified hexahistidine (His6)- and maltose binding protein (MBP)-tagged CapRelSJ46 (His6–MBP–CapRelSJ46) and either the wild-type or G24D variant of His6–Gp54Bas11 were added to the reactions. Image shown is representative of three biological replicates.
