Extended Data Fig. 7: Analysis of Gp54 homologs in related phages.
From: A bacterial immunity protein directly senses two disparate phage proteins
(a) Whole genome alignment of phages Bas11, Bas10, Bas8, Bas5 and SECΦ27. Gp54 and its homologs are labeled with purple boxes. Green and red colored blocks represent corresponding homologous regions of phages that had genome rearrangement, with the height of these blocks indicating similarities. (b) Summary of 3 independent replicates of phage spotting assay in Fig. 4b. Asterisk indicates p = 10−6 (unpaired two-tailed t-test). (c) Serial dilutions of cells producing CapRelSJ46 from its native promoter and Gp54Bas11 or Gp57Bas10 (the Gp54 homolog in Bas10) or the indicated variant from an arabinose-inducible promoter on media containing glucose or arabinose. (d) The crystal structure of Gp54Bas11 (purple) bound to CapRelSJ46 (colored by domains). Substitutions in Gp54Bas11 observed in Bas11 escape mutant (G24D) and residue I25 that is different between Gp54Bas11 and Gp57Bas10 are colored in cyan. (e) Comparison of the unbound (pink) and the CapRelSJ46-bound state (purple) of Gp54Bas11, indicating the structural changes. Residue A7V might stabilize the β-barrel core in the unbound state.
