Fig. 4: Homologues of Gp54Bas11 do not trigger CapRelSJ46.
From: A bacterial immunity protein directly senses two disparate phage proteins
a, Multiple sequence alignment of Gp54 homologues from the indicated phages. Residues that are different between the Gp54 homologue from phage Bas11 and Bas10 are labelled by red arrows. b, Serial dilutions of wild-type (ancestral or from a control population evolved without selective pressure) and the escaping clones of Bas10 spotted on lawns of cells harbouring either an EV or a plasmid producing CapRelSJ46. The corresponding genotypes of its MCP and its Gp54 homologue (Gp57Bas10) are indicated. c, Serial dilutions of cells producing CapRelSJ46 from its native promoter and the indicated MCP or its variant from an arabinose-inducible promoter on medium containing glucose or arabinose. d, As in c but with each Gp54 homologue from the indicated phages. e, As in c but with the indicated variant of Gp54Bas11.
