Extended Data Fig. 2: Gp54Bas11 binds directly to CapRelSJ46.
From: A bacterial immunity protein directly senses two disparate phage proteins
(a) Multiple sequence alignment of CapRelSJ46 and diverse CapRel homologs, with labels indicating the secondary structural elements of CapRelSJ46 and the antitoxin domain (pseudo-ZFD and anchors). The residues whose substitutions affect the activation of CapRelSJ46 by Gp54Bas11 are highlighted with stars. Alignment was adapted from previous work11. (b) Serial dilutions of cells producing CapRelSJ46 or a FLAG-tagged version from its native promoter and Gp54Bas11 or a HA-tagged version from an arabinose-inducible promoter on media containing glucose or arabinose. (c) Summary of 3 independent replicates of phage spotting assay in Fig. 2d. Asterisk indicates p = 10−18 (unpaired two-tailed t-test). (d) Serial dilutions of phage T7 spotted on lawns of cells harboring an empty vector (EV) or a plasmid producing CapRelSJ46, CapRelEbc or the chimera. Data also shown in previous work11.
