Fig. 2: Design and verification of selective epi-bit writing.
From: Parallel molecular data storage by printing epigenetic bits on DNA
a, Schematic of the principle of selective methylation. b, Design of the single-bit methylation writing assay. c, Gel-shift assay detecting different methylation states. (DNA b2: positive control of double methylated DNA b1). d, Fluorescent detection of different methylation states. e, Illustration of epi-bit reading by nanopore sequencing. f,g, Methylation calling of the nanopore signals of templates treated by M.SssI (f) and DNMT1 (g) using Nanopolish and Megalodon, respectively. The true states of methylation are shown in cartoon representations. Methylation probabilities are shown as colour intensities (f) or bar heights (g).
