Extended Data Fig. 1: The illustrations and results of the preparation of ssDNA carriers.
From: Parallel molecular data storage by printing epigenetic bits on DNA
a, The illustrations of the procedures to prepare ssDNA carriers. b, The illustrations of the DNA bricks-carrier complexes. c, The purification of the ssDNA carrier. The fluorophore modified DNA probes were used to hybridize the ssDNA carrier targets. To avoid using GelRed to dye the target DNA, the DNA ladders were individually cut as one agarose strip and subsequently soaked in GelRed solution. Then, the agarose strips of the ladders and ssDNA carriers were put together for detection under UV irradiation. The target ssDNA product was clearly observed in lane 2, and was then purified by using PCR clean up and gel extraction kit for the following methylation writing step. d, The gel results for the verification of the ssDNA carriers. (i) The gel results of the asymmetric PCR product of ssDNA carrier T960. (ii) The gel results of carrier T960 hybridized with 10, 20, 30 and 36 DNA bricks. (iii) The gel results of the asymmetric PCR product of ssDNA carrier T1200. (iv) The gel results of carrier T1200 hybridized with 8, 16, 24, 32, 40 and 48 bricks.
