Extended Data Fig. 2: The epi-bit writing tests. The epi-bit writing test on T1200 DNA carrier with 17 modification sites.
From: Parallel molecular data storage by printing epigenetic bits on DNA
The numbers of methylation sites were designed as one (117, 163, 209, 439, 485, 531), two (579–597, 603–621, 629–643, 826–830) and three (917-924-931) in the 24 nt bricks. a, The designed modification pattern. Solid and open ovals represent modified and non-modified CpG sites. b, The read coverages in nanopore sequencing along T1200. c, The methylation calling results from individual sequencing reads. d, The predicted methylation probabilities by megalodon. Red dots indicate the predesigned epi-bit 1 methylation sites. Box plots show the distribution of the data in bar plots (n = 187 for epi-bit 0, n = 17 for epi-bit 1). e, The durability of methylation storage under 25 °C and 95 °C for 1 h. f, Correlation between methylation calling results under the conditions of 25 °C and 95 °C.
