Extended Data Fig. 1: In vitro biology.
From: Glucose-sensitive insulin with attenuation of hypoglycaemia
a, Glucose-dependent hIR-A affinity of NNC2215 with no HSA in the assay. Data are mean ± SD (n = 3 independent replicates). In each set of experiments the measurements were performed with three technical replicates. aDetermined from the IC50 in the presence of 0 or 20 mM D-glucose (without HSA). b, Glucose-dependent hIR-A affinity of NNC2215a with 1.5% HSA in the assay. Data are mean ± SD (n = 3 independent replicates). In each set of experiments the measurements were performed with three technical replicates. bDetermined from the IC50 in the presence of 0 or 20 mM D-glucose (with 1.5% HSA). c, Representative displacement curves of 125I-IGF-1 from hIGF-1R for NNC2215 and human insulin in the presence of 0 or 20 mM D-glucose. Data are mean ± SD (n = 3 technical replicates). For some data points, the SD error bars are shorter than the size of the symbols. d, Glucose-dependent IGF-1R affinity of NNC2215 with no HSA in the assay. Data are mean ± SD (n = 3 independent replicates). In each set of experiments the measurements were performed with three technical replicates. cDetermined from the IC50 in the presence of 0 or 20 mM D-glucose (without HSA). e-g, Representative curves of IR (e), Akt (f) and ERK (g) phosphorylation in CHO-hIR cells induced by increasing concentrations of NNC2215 or human insulin. Data are mean (n = 2 technical replicates).
