Extended Data Fig. 1: Compound- and dose-dependent inhibitory responses to bitter compounds and amino acids.
From: Mosquito taste responses to human and floral cues guide biting and feeding
a, Schematic of base electrophysiology recording, where the recording electrode and taste stimulus are delivered separately, allowing measurement of neuronal firing before, during, and after the taste stimulus delivery. b, Example traces of S-class sensilla responding to control (paraffin oil; top), 1 mM QUI (bottom) during base recording. c, Peristimulus time histogram of base recording of S-class sensilla responding to 1 mM QUI. d, Spike rates before, during, and after exposure to 1 mM QUI, n = 6 biologically independent experiments. e, Example trace of S-class sensilla responding to 10 mM Serine. f, Peristimulus time histogram of base recording of S-class sensilla responding to 10 mM Serine. g, Spike rates before, during, and after exposure to 10 mM Serine, n = 14 biologically independent experiments. h, Example trace of S-class sensilla responding to 10 mM Lysine. i, Peristimulus time histogram of base recording of S-class sensilla responding to 10 mM Lysine. j, Spike rates before, during, and after exposure to 10 mM Lysine, n = 10 biologically independent experiments. k, Example trace of S-class sensilla responding to 10 mM Cysteine. l, Peristimulus time histogram of base recording of S-class sensilla responding to 10 mM Cysteine. Line indicates mean spike rate, and shaded region indicates ±SEM in c, f, i, and l. m, Spike rates before, during, and after exposure to 10 mM Cysteine, n = 14 biologically independent experiments. Welch’s t test (two-tailed) was used for comparison in panels d, g, j, and m. Asterisks indicate significant difference in mean spike rate, *p < 0.05, **p < 0.01 in all panels. n, Schematic of tip electrophysiology recording, where the recording electrode and taste stimulus simultaneously make contact with the taste sensillum. o-r, Example traces of S-class sensilla responses to control (TCC solvent; top), 0.1 mM (middle), and 1 mM (bottom) concentrations of Denatonium benzoate (DEN)(o), Quinine (QUI)(p), Berberine chloride (BER)(q), and Caffeine (CAF)(r). s-v, Summary of dose-dependent inhibitory responses, in comparison to spontaneous spike rate of each sensillum, for DEN, n = 15–27 (s), QUI, n = 12–27 (t), BER, n = 12–27 (u), and CAF, n = 9–27 biologically independent experiments (v). Control firing rate observed with TCC solvent alone is subtracted from tastant responses of each corresponding sensillum type in s-v. Data are presented as mean values and error bars are ±SEM in all panels.
