Extended Data Fig. 4: Explaining the multiple Apc hits.
From: Polyclonality overcomes fitness barriers in Apc-driven tumorigenesis
a, Microdissection of heterotypic tumour with detected Apc nonsense mutations overlaid. b, Possible organisation of clones that would explain sequencing results obtained in a. c, Copy number analysis summary showing relative quiescence of copy number alterations in Apchet + ENU tumours. n = 6 tumours. d, Schematic showing experimental design for mutation phasing experiment. e, Lollipop plot showing Apc mutation location and allelotypes from microdissected minor clones. n = 11 minor clones with detected Apc mutations. f, Stacked bar chart showing the fraction of tumours across different intestinal regions with mutations in particular Apc domains. n = 59 tumours in SI10, 51 in SI20, 25 in SI30, 18 in SI40, 4 in colon, and 99 tumours with no recorded location information. g, Circos plot showing combinations of Apc domain mutations between the major and minor clone pairs within polyclonal tumours.
