Extended Data Fig. 2: Tropical D. melanogaster, D. simulans and D. mauritiana strains display prominent evening peak plasticity and morning peak activity.
From: Circadian plasticity evolves through regulatory changes in a neuropeptide gene
a, Mean normalised activity of laboratory D. melanogaster (DmelCS and DmelOR), two recently-collected strains of D. melanogaster (DmelL72 and DmelL76, from the Lower Zambezi Valley), D. simulans (MD221 and MD242, from Madagascar), D. mauritiana (Dmau90 and Dmau91, from Mauritius) and laboratory D. sechellia (Dsec07, Dsec13, Dsec28 and Dsec32) under 12:12 h LD (top) and 16:8 h LD (bottom). Plots depict average activity of the last 4 days of a 7-day recording period; for 16:8 h LD, this was preceded by 7 days of 12:12 h LD. Dashed boxes highlight the pre-dawn period, 3 h before lights-on. Dashed lines highlight the average evening peak time. Error bars represent SEM. b, Evening peak time for these flies under 12:12 h LD (left) and 16:8 h LD (right). Sample sizes: DmelCS (22), DmelOR (16), DmelL72 (29), DmelL74 (46), DsimMD221 (27), DsimMD242 (34), Dmau90 (19), Dmau91 (28), Dsec07 (19), Dsec13 (20), Dsec28 (24), Dsec32 (22). c, Mean normalised activity of individual flies within this pre-dawn period. Sample sizes: DmelCS (24), DmelOR (21), DmelL72 (41), DmelL74 (61), DsimMD221 (57), DsimMD242 (52), Dmau90 (33), Dmau91 (34), Dsec07 (19), Dsec13 (82), Dsec28 (77), Dsec32 (73). d, The approximate collection sites of the D. melanogaster, D. simulans, D. mauritiana and D. sechellia strains used in a-c. For b-c, letters depict significant differences detected between strains (Wilcoxon tests for all pairwise comparisons with Bonferroni correction, P < 0.05).
