Extended Data Fig. 7: TAI profile across life cycle stages of Ectocarpus, under different RNA-seq transformations.

TAI was computed after applying different RNA-seq transformations: A, raw TPM (none); B, log2(TPM + 1); C, rank; and D, regularized log. A lower TAI marks a transcriptome composed of evolutionarily older genes and vice versa. Blue lines represent male stages while red lines represent female stages. The upper and lower limits of the boxes demarcate the s.d., based on 50,000 bootstraps.