Extended Data Fig. 9: Selective suppression of FABP5 surface expression in ER-stressed CD8+ T cells.
From: Transgelin 2 guards T cell lipid metabolism and antitumour function
a-e, WT naïve CD8+ T cells were stimulated via CD3/CD28 for 24 h and then electroporated with the indicated mRNAs. T cells were maintained under CD3/CD28 stimulation for an additional 32 h and then treated with the ER stressor TM for 16 h. a, FACS histograms and quantitative analysis of TAGLN2; b, lipid uptake, total and surface expression of: c, FABP5, d, FABP4, and e, CD36 in CD8+CD44+ T cells determined by FACS (n = 5 per condition). f-j, Pan B cells isolated from Tagln2fl/fl or Tagln2fl/flVav1Cre mice were cultured under the indicated conditions (f). FACS histograms and quantitative analysis of TAGLN2 (g), surface FABP5 (h), total FABP5 (i) and lipid uptake (j) in CD19+ B cells. (n = 3 per condition). k-o, γδ T cells isolated from Tagln2fl/fl or Tagln2fl/flVav1Cre mice were cultured under the indicated conditions (k). FACS histograms and quantitative analysis of TAGLN2 (l), surface FABP5 (m), total FABP5 (n) and lipid uptake (o) in CD3+γδ+ T cells. (n = 3 per condition). Data are presented as mean ± s.e.m. a-e,g-j,l-o, One-way ANOVA with Tukey multiple comparisons test. P < 0.05 is considered statistically significant and exact P-values are shown. The ‘n’ values represent biologically independent samples. gMFI, Geometric mean fluorescence intensity. The images in f and k were created using BioRender (https://biorender.com).
