Fig. 1: VMHBDNF neurons suppress food intake and body weight.
From: A subcortical feeding circuit linking an interoceptive node to jaw movement
a, Schematic of a coronal brain slice containing the VMH and representative example of immunolabelling for c-Fos (red) and BDNF–GFP (green) after 16 weeks of HFD of n = 3 mice. b, Quantification of c-Fos-positive and BDNF–GFP cells in the VMH of n = 3 HFD-fed and n = 3 chow-fed mice. c, Quantification of c-Fos-positive cells expressing BDNF–GFP after HFD (n = 3 mice). d, Schematic of bilateral DtA injection and timeline for mice fed chow ad lib. e,f, Weekly time course of body weights (e) and calorie intake (f) of chow-fed DtA (n = 8) and control (n = 8) mice. g, Comparison of percentage body fat between DtA (n = 8) and control (n = 8) mice. h, Timeline for mice fed HPD ad lib. i,j, Weekly time course of body weights (i) and calorie intake (j) of HPD-fed DtA (n = 8) and control (n = 8) mice. k, Comparison of percentage body fat between DtA (n = 8) and control (n = 8) mice on HPD. l,m, Body weight comparison between chow-fed DtA mice (n = 8) and control mice (n = 8) before DtA ablation and 16 weeks postsurgery (l) and HPD-fed mice (m) (n = 8 mice per group). n,o, Comparison of average daily calorie intake between chow-fed DtA and control mice at 1 and 16 weeks postsurgery (n) and HPD-fed mice (o) (n = 8 mice per group). In b, t-tests were performed using the Holm–Sidak method. Unpaired t-tests with Welch’s correction were used in g and k. Two-way repeated-measures analysis of variance (RM ANOVA) with Šídák’s multiple comparisons test was used in l–n. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Error bars indicate s.e.m. Scale bar, 200 μm. Elements (mice) in a, d and h were created using BioRender (https://biorender.com). d is adapted from ref. 56, Elsevier.
