Fig. 3: VMHBDNF neuron activity is decreased during food consumption and tuned to energy state.
From: A subcortical feeding circuit linking an interoceptive node to jaw movement
a, Schematic of a coronal brain slice with GCaMP expression and fibre placement and (below) recording timeline for energy state manipulations. b, Average photometry trace ± s.e.m. (n = 6 mice) aligned to a consumption bout of a treat pellet in mice fed chow ad lib (left) or a chow pellet (right). c, Comparison of the minimum signal between chow and treat pellet consumption (n = 6 mice). d, Heatmap of average photometry recordings per mouse (n = 6 mice) aligned to a bout of treat pellet consumption. e, Average overall photometry trace ± s.e.m. (n = 6 mice) aligned to a bout of treat pellet consumption in fasted (purple) and 4 weeks HPD-fed (beige) mice. f, Comparison of the minimum signal during treat consumption in n = 6 mice. g, Heatmap of average photometry recordings per mouse (n = 6 mice) aligned to food approach without consumption. h, Overall average overall photometry (n = 6 mice) aligned food approach without consumption in fasted (purple) and 4 weeks HPD-fed (beige) mice. i, Comparison of the maximum signal. Paired t-test was used in c, and a mixed-effects model (restricted maximum likelihood) with Holm–Šídák’s multiple comparisons test was used in f and i. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Error bars indicate s.e.m. Elements in b, e and h were created using BioRender (https://biorender.com). a is adapted from ref. 56, Elsevier. Photographs of food pellets in b,e,h are from Bio-Serv.
