Extended Data Fig. 1: Overlap of VMHBDNF neurons with Vglut2 and Sf1 and effect of TrkB inhibition. | Nature

Extended Data Fig. 1: Overlap of VMHBDNF neurons with Vglut2 and Sf1 and effect of TrkB inhibition.

From: A subcortical feeding circuit linking an interoceptive node to jaw movement

Extended Data Fig. 1

a, Representative example of coronal sections (n = 3 mice) through the VMH with in situ hybridization for BDNF (magenta) and Sf1 (white) and DAPI (blue). b, Quantification of overlap with SF1 and BDNF in dorsomedial and central VMH of n = 3 mice. c, High magnification examples of boxed areas from a with white arrows indicating BDNF only, yellow arrows Sf1 only, and green arrows BDNF and SF1 expression. d, Representative example of coronal sections (n = 3 mice) through the VMH with in situ hybridization for BDNF (magenta) and Vglut2 (green) and DAPI (blue). e, High magnification examples of overlap (indicated by white arrows) of boxed areas from d. Right, quantification of Glut2 expression in VMHBDNF neurons of 3 mice. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Error bars ± s.e.m.

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