Extended Data Fig. 8: LepR knockout in BNC2 neurons causes obesity.
From: Leptin-activated hypothalamic BNC2 neurons acutely suppress food intake
a, Body weight of female mice on chow diet post sgCtrl or sgLepr injection (N = 4 mice per group, two-way ANOVA). b, Body composition of female mice 8 weeks post-injection (N = 4 mice per group, unpaired Student’s t-test). c, Daily chow intake 8 weeks post-injection (N = 4 mice per group, unpaired Student’s t-test). d, e, EE om male mice from Fig. 5a measured by indirect calorimetry at 10–12 weeks post-injection (N = 11 mice per group, unpaired Student’s t-test). f,g, Regression analysis of EE vs. lean mass (f) and body weight (g) from e (N = 11 mice per group, ANCOVA). h,i, Locomotion for male mice from Fig. 5a (N = 11 mice per group, unpaired Student’s t-test). j,k, RER in male mice from Fig. 5a (N = 11 mice per group, unpaired Student’s t-test). l, Body weight of male mice on an HFD post sgCtrl or sgLepr injection (N = 8 sgCtrl-injected mice, N = 9 sgLepr-injected mice, two-way ANOVA). m, Body composition from l 8 weeks post-injection (N = 8 sgCtrl-injected mice, N = 9 sgLepr-injected mice, unpaired Student’s t-test). n, Daily HFD intake from l 8 weeks post-injection (N = 8 sgCtrl-injected mice, N = 9 sgLepr-injected mice, Mann-Whitney test). o, Fasting glucose levels in male mice from Fig. 5a (N = 11 mice per group, unpaired Student’s t-test). p,q, GTT of male mice from Fig. 5a at 10–12 weeks post-injection (N = 11 mice per group, unpaired Student’s t-test). r,s, ITT of male mice from Fig. 5a at 10–12 weeks post-injection (N = 6 mice per group, unpaired Student’s t-test). Data are presented as mean ± SEM. ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001. Statistical details in Source Data.
