Fig. 5: LepR knockout in BNC2 neurons causes obesity.
From: Leptin-activated hypothalamic BNC2 neurons acutely suppress food intake
a, Body weight of male mice on chow diet following sgCtrl or sgLepr injection (n = 14 mice per group; two-way ANOVA). b, Body composition of the two groups 8 weeks after virus injection (n = 11 mice per group, unpaired Student’s t-test). c, Daily chow intake 8 weeks after injection (n = 13 sgCtrl-injected mice, n = 14 sgLepr-injected mice; Mann–Whitney test). d, Food intake 3 h after PBS or leptin injection at the onset of the night dark phase in male mice (n = 7 mice per group; two-way ANOVA). e, Food intake 3 h after PBS, leptin or Sema administration at the night phase onset in male mice (n = 6 mCherry-injected mice, n = 7 dtA-injected mice; two-way ANOVA). f, Glucose concentrations at 0 and 1 h after CNO injection following 16-h overnight fasting in male mice (n = 7 mCherry-injected mice, n = 8 hM3Dq-injected mice; paired Student’s t-test or Wilcoxon test). g,h, GTT of two groups 1 h after CNO injection (n = 7 mCherry-injected mice, n = 8 hM3Dq-injected mice; Mann–Whitney test). GTT curves (g) and area under curve (AUC) (h). i,j, ITT of two groups of mice 1 h after CNO injection (n = 8 mCherry-injected mice, n = 8 hM3Dq-injected mice, unpaired Student’s t-test). ITT curves (i) and AUC (j). k, Glucose concentrations at 0 and 1 h after CNO injection after 16-h overnight fasting in male mice (n = 6 mCherry-injected mice, n = 5 hM4Di-injected mice, paired Student’s t-test). l,m, GTT 1 h after CNO injection (n = 8 mCherry-injected mice, n = 8 hM4Di-injected mice, unpaired Student’s t-test). GTT curves (l) and AUC (m). n,o, ITT 1 h after CNO injection (n = 5 mCherry-injected mice, n = 7 hM4Di-injected mice, unpaired Student’s t-test). ITT curves (n) and AUC (o). Data are presented as mean ± s.e.m. NS, not significant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.001. Statistical details in Source Data.
