Extended Data Fig. 2: Generation of BNC2-P2A-iCre knockin mouse line.
From: Leptin-activated hypothalamic BNC2 neurons acutely suppress food intake
a, Schematic of the BNC2-P2A-iCre locus. b, Sequence information of gRNAs and lssDNA. c, Genomic PCR using specific primers (N = 3 mice). d, Cre expression was assessed using a Cre-dependent mCherry viral construct. Colocalization with endogenous Bnc2 mRNA was confirmed through RNA ISH (N = 3 mice). Scale bar, 20 μm. Illustrations in a and c were created using BioRender (https://biorender.com).
