Extended Data Fig. 3: Activation of BNC2 neurons by leptin.
From: Leptin-activated hypothalamic BNC2 neurons acutely suppress food intake
a, Representative images of a patched GFP-labeled BNC2 neuron. b, Spontaneous APs of BNC2 neurons in the ARC from ad-libitum-fed adult male BNC2-Cre mice (injected with DIO-GFP). Leptin was added to the bath solution at the indicated time window. c,d, Spontaneous AP frequency (c) and RMP (d) of BNC2 neurons before, during, and after leptin application (N = 12 cells from 4 mice, Friedman test or one-way ANOVA). e, Percentage of leptin-responsive BNC2 neurons under the overnight fasting and ad-libitum-fed conditions. Data are presented as mean ± SEM. *p < 0.05; **p < 0.01. Statistical details in Source Data.
