Extended Data Fig. 7: Single-cell characterization of the proximal, middle, and distal regions of the colon in SPF, GF, and FMT mice.
From: Spatially restricted immune and microbiota-driven adaptation of the gut
a, Schematic of the different regions of the colon showing anatomical location of the middle colon in the visceral cavity (left) and the delineation of the proximal, middle, and distal colon (middle). Regions A to D as they were partitioned for scRNA-seq are then shown (right). b, UMAP of single-cell transcriptomics showing 99 annotated cell subsets. c, Number of cells of each major cell lineage captured by the single-cell dataset. d-f, UMAPs of enterocyte (d), fibroblast (e) and goblet cell (f) lineages.
