Extended Data Fig. 3: Cryo-EM reconstructions of apoB100 bound to LDLR and legobody.
From: Structure of apolipoprotein B100 bound to the low-density lipoprotein receptor
a, A second globular domain is apparent at a very low (0.05) density threshold. Superpositioned cryo-EM reconstructions of 1:2:1 (black) and 2:2:2 (white) complexes with LDL, density bridges, ridge and globular domains labelled. b–f, Cryo-EM reconstruction coloured by local resolution. All surfaces were thresholded to 0.1 and gradient coloured by local resolution 3.7–10 Å as indicated by key. b, Local refinement of the NTD of apoB100 from the 2:2:2 complex at 5.46 Å resolution. c, Non-uniform refinement of the 1:2:1 complex at 5.41 Å resolution. d,e, Local refinements of the legobody and neighbouring portions of apoB100 and LDLR (d) and the apoB100 β-barrel and LDLR β-propeller (e) at 4.18 Å and 4.83 Å resolution, respectively. f, Local refinement of portions of the legobody, apoB100 and LDLR in the vicinity of the nanobody binding site at 3.73 Å resolution. g, β-strand (left) and side chain (right) resolution of the map (transparent white) with structure (coloured by heteroatom) from the nanobody binding structure shown in f.
